Bovine Leukemia Virus (BLV) isolates genetic analysis in perinatally infected cattle at young age

The purpose of the research is to identify perinatal infection in dynamics, and assess number genetic status bovine leukemia proviruses isolated from young animals, correlations between some indicators infectious process based on gene diagnostics methods. Materials methods . We used material cattle different age groups: 1, calves (30–40 minutes after birth before colostrum 15 45 days); 2, heifers (not older than two years). Radial immunodiffusion (RID), real-time polymerase chain reaction (PCR), phylogenetic analysis were used. Results discussion An assessment given for cattle. detection rate was 4.15% (PCR) 1.09% (RID); 1.1% 0.88% (RID) heifers. A 36-fold decrease found positive dynamics (2013–2022) 14.5 0.4% with passing through 0% (2020) being at level (2022). proviral load ranged 2.02 × 104 8.38 106 GE/mL blood examined animals. BLV isolates obtained shown belong genotypes, GIV GVII (env), clade 1 (pol). assessed an overestimation by a factor three animals under years (3.83 106GE/mL) relative that 1-month-old (1.3 GE/mL), nine It important develop algorithms increase effectiveness routine tools prevent spread this retrovirus early stage, which confirmed detected over time. provirus higher calves; multiparous dairy cows nulliparous quantitative animals’ genotype those variant BLV.